Cloning vector with a blunt-end insert for use in positive...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/70 (2006.01) C12N 15/31 (2006.01) C12N 15/65 (2006.01) C12Q 1/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2429235

Vecteur de clonage comprenant une séquence nucléotidique codant pour une protéine poison et un site d'enzyme de restriction localisée dans une région codante essentielle de ladite séquence nucléotidique, ledit enzyme de restriction générant des extrémités franches et étant actif dans des conditions de ligature. Une méthode de sélection de clones recombinants. Et utilisation d'un vecteur pour la sélection de clones recombinants et pour le clonage, le séquençage ou l'amplification d'un insert d'intérêt.

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