Cloning vectors comprising a restriction site bank and the...

Details Cloning vectors comprising a restriction site bank and the... Cloning vectors comprising a restriction site bank and the...

C - Chemistry, Metallurgy

12

N

195/1.14, 195/1.

C12N 15/00 (2006.01) C12N 15/69 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01) C12N 15/78 (2006.01) C12N 15/81 (2006.01)

Patent

CA 1229315

Abstract: Cloning vectors comprising a restriction site bank and the construction thereof Vectors designed for cloning any foreign DNA material in prokaryotic and eukaryotic organisms are provided, which vectors comprise a restriction site bank containing a large number of unique restriction sites. These vectors allow cloning of large DNA fragments and complete genes without the risk of unwanted cleavage of the DNA material by restriction enzymes. Described are also methods for pro- paring the vectors and processes using same for the cloning of DNA material, for the production of polypeptides etc.

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