Cloning vectors containing selectable genetic markers for...

C - Chemistry – Metallurgy – 12 – N

Patent

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195/1.16, 195/1.

C12N 15/00 (2006.01) C12N 15/76 (2006.01)

Patent

CA 1228311

Abstract The present invention discloses novel recom- binant DNA cloning vectors for use in Streptomyces and related organisms. These novel cloning vectors comprise a) a functional origin of replication-con- taining restriction fragment of plasmid pFJ301, and b) one or more DNA segments that convey re- sistance to at least one antibiotic or colorimetric selectivity when transformed into a sensitive, restric- tionless E. coli or Streptomyces host cell that is susceptible to transformation, cell division, and cul- ture.

474081

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