Cloning vectors for cyanobacterium

Details Cloning vectors for cyanobacterium Cloning vectors for cyanobacterium

C - Chemistry, Metallurgy

12

N

195/1.16

C12N 15/00 (2006.01) C12N 15/74 (2006.01)

Patent

CA 1216531

ABSTRACT OF THE DISCLOSURE Shuttle cloning vectors, for the cloning of DNA in both E. coli bacteria and cyanobacteria (blue-green algae) are constructed using a bacterial plasmid with a polylinker containing many different restriction enzyme sites. These shuttle vectors contain an antibiotic resistance gene, an origin of replication for E. coli, an origin of replication for cyanobacteria and a polylinker with many restriction enzyme sites for the insertion of DNA fragments. Plasmid vectors generated by this procedure can be used for genetic engineering of cyanobacteria and to clone and manipulate plant genes, for the genetic engineering of plants.

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