Cloning vectors for expression of exogenous protein

Details Cloning vectors for expression of exogenous protein Cloning vectors for expression of exogenous protein

C - Chemistry, Metallurgy

12

N

195/1.16, 195/1.

C12N 15/00 (2006.01) C07K 14/61 (2006.01) C12N 15/62 (2006.01) C12N 15/70 (2006.01)

Patent

CA 1231068

Abstract The construction of a recombinant DNA cloning vector useful for expressing exogenous protein is described, which comprises (a) a DNA segment containing a functional origin of replication; (b) one or more DNA segments, each of which conveys to a transformable host cell a property useful for selection when said vector is transformed into said host cell; and (c) a DNA segment comprising a sequence that defines, in tandem, (1) the promoter of a lipoprotein expression control sequence, (2) the 5' untranslated region of a lipoprotein expression control sequence and (3) a translation start codon followed, without interposition of a portion or all of nucleotide sequence coding for endogenous protein, by a nucleotide sequence coding for an exogenous protein or by a nucleotide sequence coding for an enterokinase cleavage site to which is joined, without interruption, a nucleotide sequence coding for an exogenous protein.

428700

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