C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/101, 167/185
C12N 15/70 (2006.01) C07H 17/08 (2006.01) C12N 1/21 (2006.01) C12N 15/52 (2006.01) C12N 15/64 (2006.01) C12N 15/74 (2006.01) C12N 15/76 (2006.01) C12P 19/62 (2006.01)
Patent
CA 1339355
DNA cloning shuttle vectors, including a cosmid shuttle vector, for E. coli and Streptomyces are disclosed. Specifically, disclosed shuttle vectors pAL7002 (NRRL B-18055) and pNJ1 (NRRL B-18054) contain an E. coli origin of replication, Streptomyces replication functions, and antibiotic resistance markers for both E. coli and Streptomyces. In addition, pNJ1 contains a cos sequence. Novel 2-norerythromycin antibiotics A, B, C, and D, which were produced in a strain Streptomyces erythreus 12693-240 (NML B-18053) transformed by pNJ1 bearing DNA from Streptomyces antibioticus, are also disclosed. The present invention also provides a method for producing novel antibiotics. This method for antibiotic production is applied to the transformation of a blocked mutant of S. erythreus with genomic DNA from S. antibioticus but may be more broadly applied to genes of antibiotic-producing strains transformed into cells which are blocked in the pathway for production of a different antibiotic.
532503
Katz Leonard
Mcalpine James Bruce
Tuan James
Abbott Laboratories
Goudreau Gage Dubuc
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