Cloning vehicles for polypeptide expression in microbial hosts

Details Cloning vehicles for polypeptide expression in microbial hosts Cloning vehicles for polypeptide expression in microbial hosts

C - Chemistry, Metallurgy

12

N

195/1.14, 195/1.

C12N 15/00 (2006.01) C07H 21/04 (2006.01) C12N 1/20 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1207684

Abstract Methods and compositions are provided for expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired poly- peptide linked in reading phase with one or more functional fragments derived from an outer membrane protein gene of a gram-negative bacterium. The methods utilize such plasmids to introduce genetic capability into micro-organisms for the production of proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates therefor.

393567

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