Clostridium bifermentans dna fragment bearing genes coding...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/31 (2006.01) A01N 37/18 (2006.01) A01N 43/50 (2006.01) A01N 63/00 (2006.01) A61K 39/40 (2006.01) C07H 21/00 (2006.01) C07K 14/33 (2006.01) C07K 16/12 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2166124

A nucleotide sequence having the following properties: it includes all or part of DNA fragment Xbal of 7 kb shown in figure 4A, as obtained from plasmid pCBM1 filed with the CNCM on June 15, 1993 under no. I-1317; it hybridises with oligonucleotide probe 18A (TGT GAA GTI AAT TGT GA) and/or oligonucleotide probe 16A (TTT CAT ATI GAA GCI GTI AAT GAA GG) and/or at least one of probes 66A (ATG AAT ACI AAT ATI TTT TCI ACI AA) or 66B (TC IGG TTC ICC ATA IAT CCA TTC ATC) under stringent conditions; and it codes for a protein, polypeptide or peptide capable of participating in the toxic activity of the expression products of fragment Xbal of 7 kb against Diptera larvae and mosquito or sandfly larvae in particular. The polypeptides encoded by said sequence and their use in larvicidal compositions are also disclosed.

L'invention a pour objet une séquence nucléotidique caractérisée par les propriétés suivantes: elle comprend tout ou partie du fragment d'AND XbaI de 7 kb représente à la figure 4A, tel qu'obtenu à partir du plasmide pCBM1 déposé à la CNCM le 15 juin 1993 sous le No. I-1317; elle hybride avec la sonde oligonucléotidique 8A (TGT GAA GTI AAT TGT GA) et/ou avec la sonde oligonucléotique 16A (TTT CAT ATI GAA GCI GTI AAT GAA GG) et/ou avec au moins l'une des sondes 66A (ATG AAT ACI AAT ATI TTT TCI ACI AA) ou 66B (TC IGG TTC ICC ATA IAT CCA TTC ATC) dans des conditions stringentes; elle code pour une protéine, un polypeptide ou un peptide ayant la capacité de participer à l'activité toxique des produits d'expression du fragment XbaI de 7 kb vis-à-vis de larves de Diptères et en particulier de larves de Moustiques ou de Simulies. Elle vise aussi les polypeptides codés par cette séquence et leur utilisation dans des compositions larvicides.

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