Clostridium perfringens vaccine

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/31 (2006.01) A61K 39/08 (2006.01) A61K 39/116 (2006.01) A61K 39/295 (2006.01) C07K 14/33 (2006.01) C12N 1/21 (2006.01) C12N 1/36 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2235445

The present invention relates to detoxified immunogenic derivatives of Clostridium perfringens -toxin or an immunogenic fragment thereof that have as a characteristic that they carry a mutation in the .beta.-toxin amino acid sequence, not found in the wild-type .beta.-toxin amino acid sequence. The invention also relates to genes encoding such .beta.-toxins, as well as to expression systems expressing such .beta.-toxins. Moreover, the invention relates to bacterial expression systems expressing a native .beta.-toxin. Finally, the invention relates to vaccines based upon detoxified immunogenic derivatives of Clostridium perfringens .beta.-toxin, and methods for the preparation of such vaccines.

La présente invention concerne des dérivés immunogènes détoxifiés de la toxine de Clostridium perfringens ou un fragment immunogène de cette dernière qui ont pour caractéristique d'être porteurs d'une mutation au niveau de la séquence d'acides aminés de la toxine bêta, non présente dans la séquence d'acides aminés de la toxine bêta de type sauvage. L'invention concerne également les gènes qui codent pour ces toxines bêta de même que les systèmes d'expression exprimant ces toxines bêta. De plus, l'invention concerne des systèmes d'expression exprimant une toxine bêta indigène. Enfin, l'invention concerne des vaccins produits à partir de dérivés immunogènes détoxifiés de la toxine bêta de Clostridium perfringens et des méthodes pour la préparation de ces vaccins.

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