Cloth enzyme immunoassay

Details Cloth enzyme immunoassay Cloth enzyme immunoassay

G - Physics

01

N

167/129, 167/44

G01N 33/545 (2006.01) A61K 39/39 (2006.01) A61K 39/44 (2006.01)

Patent

CA 2019048

ABSTRACT OF THE DISCLOSURE Novel devices and techniques for carrying out immunoassay techniques on antibodies, antigens or haptens are provided here- in. The device is a macroporous hydrophobic synthetic polymer cloth having antibodies or antigens directly adsorbed therein and directly absorbed and immobilized thereon. The cloth has a thickness more than 200µm and having spaces between fibres exceeding 20µm in diameter, and preferably has a Frazier Air permeability, in CFM/ft2 at 0.5" H2O of from 215 to 750 for thickness of from 11 to 40 mils, the cloth thereby having a structure such that it can accommodate a large volume of liquid per surface area thereof, that it has a large surface area, and that it has minimum flow resistance. In addition, in two alter- native embodiments: antibodies may be directly adsorbed therein and directly absorbed and immobilized thereon, and specific anti- gens from a selected test sample, may then be captured by the adsorbed, absorbed and immobilized antibodies, to be detected conventionally; or antigens may be directly adsorbed therein and directly absorbed and immobilized thereon, and specific anti- bodies from a selected test sample, may be captured by the adsorbed, absorbed and immobilized antigens, to be detected con- ventionally.

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