Co-expression of proteins

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/425 (2006.01) C07K 16/16 (2006.01) C07K 16/38 (2006.01) C12N 15/29 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2358548

The subject invention pertains to materials and methods for transformed plants and plant tissues that are capable of expressing high levels of stable proteins which are localized as protein bodies within the plant cell. Transformed plants co-expressing high levels of both the 15kD and 10kD zein proteins are disclosed which accumulate to high levels as protein bodies in the vegetative tissue of the plant. Transformed plants co-expressing the 15kD and 10kD zein proteins are useful for providing forage crops containing increased levels of sulfur containing amino acids, such as methionine, in the diet of animals that normally feed on such crops. Also contemplated by the subject invention are transformed plants or plant tissue comprising stable protein bodies which contain heterologous proteinaceous material. In one embodiment, a stable protein body is expressed in a plant or plant tissue as a fusion protein comprising a zein protein and an operably linked protein or peptide. The protein bodies provided in the present invention are resistant to rumin digestion or environmental degradation.

La présente invention concerne des matériaux et des procédés pour des plantes transformées et pour des tissus de plantes qui permettent d'exprimer de fortes teneurs en protéines stables qui sont localisées sous forme de corps protéiques dans la cellule de la plante. L'invention concerne des plantes transformées co-exprimant des fortes concentrations de protéines de zéine 15kD et 10 kD qui s'accumulent sous forme de taux élevés de corps protéiques dans le tissu végétatif de la plante. Les plantes transformées co-exprimant les protéines de zéine 15kD et 10kD permettent de constituer des cultures fourragères contenant des taux accrûs d'acides aminés à base de soufre, comme la méthionine, dans l'alimentation des animaux nourris par ces cultures. L'invention concerne également des plantes ou des tissus de plantes transformés comprenant des corps protéiques stables qui renferment un matériau protéinique hétérologue. Selon un mode de réalisation, un corps protéique stable est exprimé dans une plante ou un tissu de plante sous forme d'une protéine de fusion comprenant une protéine de zéine et une protéine ou un peptide à liaison opérationnelle. Les corps protéiques selon l'invention résiste à la dégradation par l'environnement ou par la digestion du ruminant.

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