Co-expression system for large functional proteins

Details Co-expression system for large functional proteins Co-expression system for large functional proteins

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) C07K 14/705 (2006.01) C12N 15/11 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2385110

The present invention provides a system and method for expressing functional ABC (ATP- binding cassette) proteins in a host cell. A system comprises two or more expression vectors each comprising a nucleic acid sequence encoding a region of an ABC transporter gene including one or more functional domains and means for expressing the nucleic acid sequence. Each expression vector comprises a nucleic acid sequence that encodes a dissimilar part of the ABC transporter gene as that contained in the other expression vectors but when taken together comprise the full ABC transporter gene. Co-transfection of the expression vectors into a host cell provides co-expression of each of the parts of the protein which assemble to form an ABC transporter protein having functional characteristics of the full-length protein.

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