Coamplification of target nucleic acids using volume...

Details Coamplification of target nucleic acids using volume... Coamplification of target nucleic acids using volume...

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2158486

Multiple target nucleic acids are amplified using polymerase chain reaction in the presence of a nonionic, polymeric volume exclusion agent. The amplification efficiency of low copy target nucleic acids is increased in the presence of the volume exclusion agent even though reduced primer levels are used. In this manner, amplification efficiency of a given target nucleic acid can be more readily manipulated.

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