Coanda effect gene delivery instrument and method of use

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 3/00 (2006.01) A61M 37/00 (2006.01) C12M 1/00 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2220648

An apparatus (8) is disclosed for the genetic transformation of organisms by accelerated particle mediated transformation. Foreign genes are introduced into cells by coding on carrier particles (55') which are physically accelerated into the cells by positioning the carrier particles (8) on the external surface of a carrier ribbon (56) which is wound on a cartridge (14), the carrier ribbon (56) having an exposed portion. The carrier particles (55') on the exposed portion of the ribbon (56) are displaced and accelerated toward an exit port (52) by a high pressure stream of helium gas. By rotating the ribbon (56), a continuous supply of carrier particles (8) can be produced and hence large target areas can be transformed without the need to replace the particle carrier. Near the exit port (52), the gaseous stream is diverted through use of the Coanda effect to divert the gas stream away from a target area.

L'invention décrit un dispositif (8) conçu pour transformer génétiquement des organismes par transformation induite par accélération de particules. Des gènes étrangers sont introduits dans des cellules par codage sur des particules vecteurs (55') qui sont physiquement accélérées à l'intérieur des cellules par positionnement des particules vecteurs (8) sur la surface extérieure d'un ruban vecteur (56) enroulé sur une cartouche (14), lequel ruban a une partie découverte. Les particules (55') se trouvant sur la partie découverte du ruban (56) sont déplacées et accélérées vers un orifice de sortie (52) par un courant à haute pression hélium gazeux. Grâce à la rotation du ruban (56), il est possible de fournir en continu des particules vecteurs (8) et, donc, de transformer de grandes zones cibles sans qu'il soit nécessaire de remplacer le vecteur particulaire. Près de l'orifice de sortie (52), le courant gazeux est dévié loin d'une zone cible par utilisation de l'effet Coanda.

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