Coated cells and their use

Details Coated cells and their use Coated cells and their use

G - Physics

01

N

167/45, 195/34.1

G01N 33/556 (2006.01) C12N 11/00 (2006.01) G01N 33/543 (2006.01) G01N 33/554 (2006.01)

Patent

CA 1197777

ABSTRACT OF THE DISCLOSURE Heterobifunctional reagents are used to conjugate molecules or macromolecular structures (e.g., antigens or antibodies) to membranes of non-nucleated or nucleated cells, or to liposomes, these cells being useful in highly sensitive hemolytic or immuno- cytoadherence assays to detect picogram quantities of antibodies or antigens. For example, a conjugate of an animal cell or liposome is conjugated on the surface thereof via desulfide bonds, with one or more molecules or macromolecular structures. The sulfide bonds are derived from a heterobifunctional reagent. Also disclosed is a process conjugating a thiol-con- taining molecule or macromolecular structure to an animal cell or liposome, as well as an assay method and a kit using same.

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