Codon optimisation for expression in retrovirus packaging cells

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/86 (2006.01) A61K 48/00 (2006.01) C07K 14/155 (2006.01) C07K 14/16 (2006.01) C12N 5/10 (2006.01) C12N 7/04 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2404129

A method of producing a replication defective retrovirus comprising transfecting a producer cell with the following: iii) a retroviral genome; iv) a nucleotide sequence coding for retroviral gag and pol proteins; and iii) nucleotide sequences encoding other essential viral packaging components not encoded by the nucleotide sequence of (ii); characterised in that the nucleotide sequence coding for retroviral gag and pol proteins is codon optimised for expression in the producer cell.

L'invention concerne un procédé de production d'un rétrovirus défectif comprenant la transfection d'une cellule productrice avec: iii) un génome rétroviral; iv) une séquence nucléotidique codant pour des protéines gag et pol rétrovirales; et iii) des séquences nucléotidiques codant pour d'autres composés d'encapsidation virale essentiels qui ne sont pas codés par la séquence nucléotidique de (ii). Ce procédé est caractérisé en ce que la séquence nucléotidique codant pour les protéines gag et pol rétrovirales est optimisée au niveau du codon en vue de son expression dans la cellule productrice.

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