Codon optimized il- 15 and il- 15r-alpha genes for...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/67 (2006.01) A61K 38/17 (2006.01) A61K 38/20 (2006.01) C07K 14/54 (2006.01) C07K 14/715 (2006.01)

Patent

CA 2636111

The present invention provides for nucleic acids improved for the expression of interleukin-15 (IL-15) in mammalian cells. The invention further provides for methods of expressing IL-15 in mammalian cells by transfecting the cell with a nucleic acid sequence comprising a codon optimized IL-15 sequence. The present invention further provides expression vectors, and IL-15 and IL 15 receptor alpha combinations (nucleic acid and protein) that increase IL-15 stability and potency in vitro and in vivo. The present methods are useful for the increased bioavailability and biological effects of IL-15 after DNA, RNA or protein administration in a subject (e.g. a mammal, a human).

Acides nucléiques améliorés pour l'expression de l'interleukine-15 (IL-15) dans des cellules mammaliennes. Également, procédés relatifs à l'expression de l'IL-15 dans des cellules mammaliennes par transfection de cellule avec une séquence nucléotidique codant une séquence IL-15 améliorée. Également, vecteurs d'expression, et combinaisons ' IL-15 et IL-15R-ALPHA (acide nucléique et protéine) augmentant la stabilité et la puissance de l'IL-15 in vitro et in vivo. Les procédés décrits sont utiles pour accroître la biodisponibilité et les effets biologiques de l'IL-15 après l'administration d'ADN, d'ARN ou de protéine à un sujet (par exemple, mammifère, humain)

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