Cold shock protein, gene coding therefor, promoter for gene...

C - Chemistry – Metallurgy – 12 – N

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530/7.1, 195/1.2

C12N 15/72 (2006.01) C07K 14/245 (2006.01) C12N 1/21 (2006.01) C12N 15/11 (2006.01) C12N 15/31 (2006.01) C12N 15/63 (2006.01) C12N 15/70 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2009917

The major cold shock protein of E. coli, which comprises a nucleotide sequence encoding cs7.4 with functional equivalents thereof, along with a nucleic acid molecule having a promoter sequence which is capable of demonstrating transcription of a gene at a temperature below normal growth temperature of E. coli. Consequently, construct and DNA sequence in which include the gene encoding cs7.4 protein, a promoter designed to drive cs7.4 protein, a promoter designed to drive cs7.4 expression, and the cspA gene are disclosed. Also, contemplated are transformed competent hosts and transgenic plants. The invention also provides for various applications and method in protein synthesis.

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