Collagen scaffold modified by covalent grafting of adhesion...

A - Human Necessities – 61 – L

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Details

A61L 27/34 (2006.01) A61L 27/56 (2006.01) A61L 31/04 (2006.01) A61L 31/16 (2006.01)

Patent

CA 2692005

The invention relates to materials and methods for improving the biological properties and electromechanical performance of collagen scaffolds used for cell transplantation, comprising the fixation of biocompatible reagents and adhesion molecules which control cell adhesion, apoptosis, survival and/or differentiation simultaneously. The grafting of adhesion molecules to collagen matrices renders same suitable for use in vascular and cardiothoracic surgery/medicine, as well as in cell therapy for the heart and in artificial heart muscle engineering. The invention also relates to a simple method for grafting and optimising the presentation of adhesion peptides or biological agents when the scaffold is made from collagen and/or contains accessible thiol, amine or carboxyl groups.

La présente invention fournit des matériaux et des méthodes pour améliorer les propriétés biologiques et les performances électromécaniques des échafaudages de collagène utilisés pour la transplantation cellulaire. Ceci est obtenu par fixation du support par des réactifs biocompatibles et par la fixation de molécules d'adhésion qui contrôlent à la fois l'attachement des cellules, l'apoptose, la survie et/ou la différenciation. Le greffage de molécules d'adhésion sur des matrices collagéniques conduit à des applications en médecine/chirurgie cardio-thoracique et vasculaire ainsi qu'en thérapie cellulaire dans le coeur ou pour l'ingénierie d'un myocarde artificiel. Cette invention fournit également une méthode simple pour greffer et optimiser la présentation des peptides d'adhésion ou agents biologiques si l'échafaudage est fait de collagène et/ou contient des groupements accessibles thiols, aminés ou des groupes carboxyles.

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