Colon cell and colon cancer cell associated nucleic acid...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) C07K 7/06 (2006.01) C07K 7/08 (2006.01) C07K 14/705 (2006.01) C07K 16/30 (2006.01) C12N 15/63 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2229028

This invention relates to isolated protein and to peptides which are found on the surface of colon cells and colon cancer cells, as well as to nucleic acid molecules encoding said protein and peptides. The protein and peptides bind to tumor-associated antibodies, such as mAb A33. The monomeric protein has a molecular weight of about 43 kD as determined by SDS gel electrophoresis under non-reducing conditions. In addition, this invention relates to the use of said nucleic acid molecules, protein, in monomeric or multimeric form, and to antibodies to said peptides in diagnostic, screening and therapeutic methods. This invention further relates to antibodies specific for said protein, in monomeric or multimeric form, and to antibodies to said peptides.

Protéine isolée et peptides que l'on trouve sur la surface des cellules du colon et des cellules cancéreuses du colon, ainsi que molécules d'acides nucléiques codant ladite protéine et lesdits peptides. Ladite protéine et lesdits peptides se lient à des anticorps associés à la tumeur, tels que mAb A33. La protéine monomère possède un poids moléculaire d'environ 43 kD tel que déterminé par électrophorèse sur gel SDS dans des conditions de non-réduction. De plus, la présente invention concerne l'utilisation desdites molécules d'acides nucléiques, de ladite protéine, sous forme monomère ou multimère, et des anticorps dirigés contre lesdits peptides dans des procédés diagnostiques, de criblage et thérapeutiques. La présente invention concerne en outre des anticorps spécifiques de ladite protéine, sous forme monomère ou multimère, et des anticorps dirigés contre lesdits peptides.

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