Color marker for clonings in streptomyces lividans

Details Color marker for clonings in streptomyces lividans Color marker for clonings in streptomyces lividans

C - Chemistry, Metallurgy

12

N

195/1.15

C12N 15/00 (2006.01) C07K 14/36 (2006.01) C12N 1/21 (2006.01) C12N 15/52 (2006.01) C12N 15/65 (2006.01) C12N 15/76 (2006.01)

Patent

CA 1288071

- 1 - HOE 86/F 199 Abstract of the disclosure: Intensely crimson colonies are obtained from Streptomyces coelicolor DSM 3030 by total digestion of the total DNA, cloning into a suitable vector, and transformation of Streptomyces lividans. Re-isolation of the plasmid DNA and cutting with BamHI results in fragments about 3.4 to 9 kb in length, on which is located the gene which deter- mines production of the coloring agent. This gene is suitable as marker, in particular as inactivation marker, in Streptomycetes.

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