Colorimetric assay for bilirubin

Details Colorimetric assay for bilirubin Colorimetric assay for bilirubin

G - Physics

01

N

150/11, 150/14

G01N 33/48 (2006.01) G01N 33/72 (2006.01) G01N 33/80 (2006.01) G01N 33/86 (2006.01)

Patent

CA 1094432

Abstract of the Disclosure A colorimetric assay method for the detection of bilirubin in various liquid samples, particularly biological liquids. A multi-zone analytical element based on this assay method is also disclosed. The assay method employs an inter- active mordant composition for bilirubin having one or more binding sites for bilirubin, such composition comprising a hydrophobic organic matrix and at least one charge-bearing cationic group. Upon mordanting of bilirubin by the interactive mordant composition, the mordanted bilirubin exhibits an absorption peak shift of at least 10 nm, and at least a 50% increase in molar extinction coefficient.

292579

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