C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/28 (2006.01) G01N 31/22 (2006.01)
Patent
CA 2098189
ABSTRACT OF THE DISCLOSURE A method to determine the presence or amount of peroxidase or hydrogen peroxide employing a sulfonephthalein dye or a phthalein dye having a major absorbance peak in the visible region, preferably at a wavelength from between about 500 nanometers to about 700 nanometers, and which absorbance peak is subject to a degree of oxidative extinguishment without strong alkali treatment, is disclosed. The method is performed by forming a reaction system comprising such sulfonephthalein dye or phthalein dye, and a test sample containing an unknown amount of (i) peroxide, (ii) a peroxidase, (iii) a compound which is capable of being oxidized to generate hydrogen peroxide, or (iv) a catalyst which participates in such oxidation, wherein the hydrogen peroxide causes peroxidase-catalyzed extinguishment of the absorbance peak of such dye whereby the unknown amount under determination can be determined by monitoring such extinguishment.
Salt Steven D.
Siegel Neal A.
Abbott Laboratories
Swabey Ogilvy Renault
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