Colorimetric immunoassay on solid surface

Details Colorimetric immunoassay on solid surface Colorimetric immunoassay on solid surface

G - Physics

01

N

167/44

G01N 33/58 (2006.01) G01N 33/543 (2006.01) G01N 33/577 (2006.01) G01N 33/74 (2006.01)

Patent

CA 1223813

Abstract of the Disclosure A method for the colorimetric immunoassay of antigen or antibody to be detected in a biologically derived fluid sample using a solid surface. Preferably, the surface is an impermeable surface of a dipstick. To detect antigen in the sample, a dipstick containing bound antibody is contacted with the sample and with a soluble enzyme-labeled antibody, to cause the antigen to bind to both the immobilized antibody and the labeled antibody on the surface. After separation of unreacted reagents, the solid surface is contacted with a substrate which converts from a soluble form to an insoluble colored form in the presence of the enzyme and deposits upon the surface of the solid surface. The solid surface is separated from the solution. The sandwich immunoassay technique may also be employed to detect antibody in the sample by reversing the roles of the antigen and antibody. The system is also applicable to a competitive binding technique.

453528

Affiliated with

Also associated with

Rating

Colorimetric immunoassay on solid surface does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Colorimetric immunoassay on solid surface, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Colorimetric immunoassay on solid surface will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1334785