Combination reagent holding and test device

Details Combination reagent holding and test device Combination reagent holding and test device

G - Physics

01

N

G01N 33/86 (2006.01) B01L 3/00 (2006.01) G01N 33/49 (2006.01) G01N 33/50 (2006.01) G01N 33/554 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2220574

A test cartridge for use in an assay system for the assay of a liquid sample comprises a housing (60) through which the liquid sample flows during the assay. The housing (60) defines a holding chamber (61) for holding the sample (11) and a test chamber (63), wherein the holding chamber (61) and test chamber (63) are separated by a pierceable member (70) having a cut therein. In the test chamber (63) there is a partition member (6) comprising at least one reagent, and having an opening (9) therethrough. A transfer member (40) is mounted in the test chamber (63) so that it can be moved towards and pierce the pierceable member (70) and contact the liquid sample (11) in the holding chamber (61). When the transfer member (40) has moved through the cut in the pierceable member (70) and contacted the liquid sample (11), a negative pressure is applied to the test chamber (63). Liquid sample (11) then moves through the transfer member (40), into the test chamber (63) and through the opening (9) in the partition member (6).

L'invention porte sur une cartouche utilisée dans un système d'essai pour effectuer l'analyse d'échantillons liquides, comprenant un boîtier (60) traversé par le liquide pendant l'analyse. Le boîtier comporte une chambre réceptrice (11) contenant l'échantillon (11) et une chambre d'essai (63), séparées l'une de l'autre par un élément perçable (70) où est pratiquée une incision., La chambre d'essai (63) comporte un élément de séparation (6) comportant au moins un réactif et percé d'une ouverture (9). Un élément de transfert (40) est placé dans la chambre d'essai (63) de manière à pouvoir se déplacer vers l'élément perçable (70), le percer, et entrer en contact avec l'échantillon liquide présent dans la chambre (61). Lorsque l'élément de transfert (40) ayant traversé l'incision pratiquée dans l'élément perçable (11) est entré en contact avec l'échantillon liquide, on met la chambre d'essai (63) en dépression. L'échantillon liquide (11) traverse alors l'élément de transfert (40), et pénètre dans la chambre d'essai (63) et dans l'élément de séparation (6) en passant par l'ouverture (9).

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