Combinatorial fluorescence energy transfer tags and uses...

C - Chemistry – Metallurgy – 07 – H

Patent

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Details

C07H 21/04 (2006.01) C07B 61/00 (2006.01) C07H 21/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/542 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2421582

This invention provides a combinatorial fluorescence energy transfer tag which comprises a plurality of fluorescent molecules, comprising one or more energy transfer donor and one or more energy transfer acceptor, linked through a molecular scaffold wherein the fluorescent molecules are separated along the scaffold to produce a unique fluorescene emission signature. The invention further provides for the use of said tags in multi-component analyses, including multiplex biological analyses.

L'invention concerne un indicateur de transfert d'énergie de fluorescence combinatoire comprenant plusieurs molécules fluorescentes, un ou plusieurs donneurs de transfert d'énergie et un ou plusieurs accepteurs de transfert d'énergie, liés via un squelette moléculaire sur lequel les molécules fluorescentes sont espacées afin de produire une signature unique par émission de fluorescence. L'invention concerne aussi l'utilisation de ces indicateurs dans des analyses à composants multiples, y compris dans des analyses biologiques multiplex.

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