C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 1/24 (2006.01) B01L 3/14 (2006.01) G01N 9/30 (2006.01) G01N 33/49 (2006.01) G01N 33/543 (2006.01)
Patent
CA 2068220
2068220 9107648 PCTABS00005 Disclosed is a centrifuge tube (10) containing a porous selection means (21) for selectively separating and recovering biological substances from gels, broths, or solutions containing the same, and thus concentrating them, said tube comprising separate upper (20) and lower (30) containers and means for joining the two containers to form a unitary device, the bottom of said upper container (20) comprising a porous selection means (21), preferably a membrane containing a particulate substance which will selectively bind the biological substances while allowing unbound substances to pass through from the upper (20) to lower container (30) while being centrifuged. A method for recovering said biological substances in high concentration using said centrifuge tube is also provided, comprising introducing said gels, broths, or solutions containing the desired biological substances, centrifuging the same until the desired substance is bound to the selection means, and eluting the bound material from the selection means to recover said substance in concentrated form.
Goldberg Bruce S.
Nochumson Samuel
Fmc Corporation
Osler Hoskin & Harcourt Llp
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