Combined hairpin-antisense compositions and methods for...

Details Combined hairpin-antisense compositions and methods for... Combined hairpin-antisense compositions and methods for...

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) A61K 31/713 (2006.01) C07H 21/00 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2681323

A nucleotide construct comprising a nucleotide sequence that forms a stem and a loop, wherein the loop comprises a nucleotide sequence that modulates expression of a target, wherein the stem comprises a nucleotide sequence that modulates expression of a target, and wherein the target modulated by the nucleotide sequence in the loop and the target modulated by the nucleotide sequence in the stem may be the same or different. Vectors, methods of regulating target expression, methods of providing a cell, and methods of treating conditions comprising the nucleotide sequence are also disclosed.

L'invention concerne un produit de construction de nucléotide comprenant une séquence de nucléotides qui forme une tige et une boucle, la boucle comprenant une séquence de nucléotides qui module l'expression d'une cible, la tige comprenant une séquence de nucléotides qui module l'expression d'une cible, et la cible modulée par la séquence de nucléotide dans la boucle et la cible modulée par la séquence de nucléotides dans la tige pouvant être identiques ou différentes. L'invention concerne également des vecteurs, des procédés de régulation de l'expression d'une cible, des procédés d'obtention d'une cellule et des procédés de traitement d'affections comprenant la séquence de nucléotide.

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