Combined polynucleotide sequences as discrete assay endpoints

Details Combined polynucleotide sequences as discrete assay endpoints Combined polynucleotide sequences as discrete assay endpoints

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2402271

Methods are provided for determining the relative amounts of individual polynucleotides in a complex mixture. The polynucleotides, after fluorescent labeling, are contacted under hybridization conditions with an array having element disposed at discrete locations on a substrate. The elements comprise two or more distinct polynucleotides that are combined prior to arraying. The level of fluorescence associated with each element provides a measure of its relative amount in the mixture.

L'invention concerne des procédés pour déterminer les quantités relatives de polynucléotides individuelles dans un mélange complexe. Après marquage fluorescent, les polynucléotides sont mises en contact dans des conditions d'hybridation avec un échantillon ayant des éléments disposés en des points discrets sur un substrat. Ces éléments comprennent deux polynucléotides distinctes ou plus, combinées avant l'échantillonnage. Le niveau de fluorescence associée à chaque élément fournit une mesure de sa quantité relative dans ledit mélange.

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