Commercial production of avidin in plants

A - Human Necessities – 01 – H

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A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/46 (2006.01) C07K 14/465 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2232736

A method for commercial production of avidin entails heterologous expression of the protein in plants, in native conformation, at an expression level such that avidin represents at least 0.1 % of total extracted protein. A genetic map of the integration locus allows for the identification of the avidin- expressing plant. Genetic loci on a plant chromosome are revealed that support high levels of avidin expression and that can be used as a site of integration for high level expression of other genes of interest.

L'invention concerne un procédé de production commerciale de l'avidine, qui consiste en une expression hétérologue de la protéine dans des plantes, en conformation native, à un niveau d'expression tel que l'avidine représente au moins 0,1 % de la protéine extraite totale. Une carte génétique du locus d'intégration permet d'identifier la plante exprimant l'avidine. Le procédé permet d'identifier sur un chromosome de plante les loci génétiques qui offrent des niveaux élevés d'expression pour l'avidine et que l'on peut utiliser comme site d'intégration pour l'expression, à un niveau élevé, d'autres gènes présentant de l'intérêt.

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