Commercial production of chymosin in plants

Details Commercial production of chymosin in plants Commercial production of chymosin in plants

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/64 (2006.01) C12N 15/12 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2381438

A method for the commercial production of chymosin which involves the recombinant expression of chymosin in plant seeds, that enables chymosin to accumulate to at least 0,5 % (w/w) of total seed protein is described. An improved method for the isolation of chymosin from the seed is also described.

L'invention porte sur un procédé visant à produire de la chymosine à un niveau industriel et comprenant l'expression par recombinaison de la chymosine dans les graines des plantes, ce qui permet à la chymosine d'accumuler jusqu'à au moins 0,5 % (en poids) de la protéine brute des graines. L'invention porte également sur un procédé consistant à isoler la chymosine des graines.

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