C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 9/02 (2006.01) C12N 15/53 (2006.01)
Patent
CA 2343734
Expression of laccase in plants at commercial levels of production is provided. The laccase gene vectors may include signal sequences directing expression to the cell wall of the plant, and may additionally include sequences targeting expression to the endoplasmic reticulum of the plant cell. Expression of laccase may also be directed to the seed of the plant. The plant having the laccase gene may additionally contain a substance that is a mediator of laccase delignification. Methods of improving the process of introducing DNA into plants via <i>Agrobacterium</i> are also provided.
L'invention se rapporte à l'expression de la laccase dans des plantes, à des niveaux de production commerciaux. Les vecteurs du gène de la laccase peuvent inclure des séquences-signal dirigeant l'expression vers la paroi cellulaire de la plante. Ils peuvent inclure en outre des séquences ciblant l'expression vers le réticulum endoplasmique de la cellule végétale. L'expression de la laccase peut également être dirigée vers la graine de la plante. La plante possédant le gène de la laccase peut en outre contenir une substance servant de médiateur de la délignification de la laccase. L'invention concerne des procédés pouvant améliorer le processus d'introduction de l'ADN dans des plantes par l'intermédiaire d'<i>Agrobacterium</i>.
Hood Elizabeth
Howard John
Jilka Joseph
Genencor International Inc.
Prodigene Inc.
Torys Llp
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