Commercial production of proteases in plants

Details Commercial production of proteases in plants Commercial production of proteases in plants

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/04 (2006.01) C12N 9/48 (2006.01) C12N 9/76 (2006.01) C12N 15/55 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2333146

Production of proteases in plants is set forth, whereby heterologous DNA encoding the protease is introduced into the plant and expression of the protein achieved. By such methods, expression is achieved in plants wherein the plant cell is not damaged, the protein can be recovered without contamination by other proteases, and can be expressed at levels such that commercial production of the enzyme is obtained. Expression levels can be at 0.1 % of total soluble protein of the plant, or higher.

L'invention concerne la production commerciale de protéases dans des végétaux. On introduit dans le végétal l'ADN hétérologue codant pour la protéase, ce qui permet d'obtenir l'expression de la protéine. Ces méthodes permettent d'obtenir l'expression dans des végétaux sans endommager les cellules végétales, de récupérer la protéine sans qu'elle soit contaminée par d'autres protéases et d'exprimer la protéine à des taux permettant de produire l'enzyme en quantités commerciales. Les taux d'expression peuvent atteindre ou dépasser le niveau de 0,1 % de la protéine soluble totale du végétal.

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