Compact optical detection system for a microfluidic device

Details Compact optical detection system for a microfluidic device Compact optical detection system for a microfluidic device

G - Physics

01

N

G01N 21/64 (2006.01) B81B 1/00 (2006.01)

Patent

CA 2603759

An optical detection system for a microfluidic device and a dry-focus microfluidic device compatible with the compact optical detection system are described. The system includes an LED; means for collimating light emitted by the LED; an aspherical, fused- silica objective lens; means for directing the collimated light through the objective onto a microfluidic device; and means for detecting a fluorescent signal emitted from the microfluidic device. The working distance between the objective and the device allows light from an external LED or laser to be brought in along a diagonal path to illuminate the microfluidic device. The dry-focus microfluidic device includes multiple channels and multiple closed optical alignment marks having curved walls. At least one of the channels is positioned between at least two of the marks. The marks are illuminated for alignment and focusing purposes by light brought in on a diagonal path from an external white LED.

L'invention porte sur un système optique de détection pour dispositif de microfluidique et sur un dispositif microfluidique à foyer sec compatible avec ledit système lequel comporte: une DEL; un moyen de collimation de la lumière émise par la DEL; un objectif asphérique de silice fondue; un moyen dirigeant la lumière collimatée via l'objectif sur un objet microfluidique; et un moyen de détection du signal fluorescent émis par le dispositif microfluidique. La distance de travail séparant l'objectif du dispositif permet d'amener la lumière d'une DEL ou d'un laser externes via une trajectoire diagonale sur le dispositif microfluidique. Le dispositif microfluidique à foyer sec comporte plusieurs canaux et plusieurs repères d'alignement optique fermés à parois courbes. L'un au moins de ces canaux se trouve entre deux de ces repères. Les repères sont éclairés à des fins d'alignement et de focalisation par la lumière arrivant en diagonale de la DEL.

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