Comparative fluorescence hybridization to nucleic acid arrays

Details Comparative fluorescence hybridization to nucleic acid arrays Comparative fluorescence hybridization to nucleic acid arrays

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2206102

The present invention provides methods of determining relative copy number of target nucleic acids and precise mapping of chromosomal abnormalities associated with disease. The methods of the invention use target nucleic acids immobilized on a solid surface, to which a sample comprising two sets of differentially labeled nucleic acids are hybridized. The hybridization of the labeled nucleic acids to the solid surface is then detected using standard techniques.

Des procédés permettent de déterminer le nombre de copies relatif d'acides nucléiques cibles et la cartographie précise d'anomalies chromosomiques liées à des maladies. Ces procédés consistent a utiliser des acides nucléiques cibles, immobilisés sur une surface solide, avec lesquels on hybride un échantillon contenant deux jeux d'acides nucléiques marqués de façon différente. On détecte ensuite par des techniques classiques l'hybridation des acides nucléiques marqués sur cette surface solide.

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