Comparative multiple analyte assay

G - Physics – 01 – N

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G01N 33/558 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2677977

Methods for measuring the relative amount of two or more analytes of interest in a fluid sample, as well as kits useful in the methods, are disclosed. The methods involve assays that utilize a solid phase apparatus with a membrane having an application point and at least two sample capture zones having sample capture reagents; analyte binding particles or analyte coated particles; and assessment of a ratio of such particles arrested in capture zones, wherein the ratio is equal to, or inversely equal to, the relative amounts of the analytes of interest in the fluid sample.

L'invention concerne des procédés permettant de mesurer la quantité relative de deux ou plus analytes d'intérêt dans un échantillon de fluide, de même que des kits utiles dans les procédés. Les procédés impliquent des dosages qui utilisent un appareil en phase solide pourvu d'une membrane munie d'un point d'application et d'au moins deux zones de capture d'échantillon possédant des réactifs de capture d'échantillon ; des particules de liaison d'analyte ou des particules enduites d'analyte ; et l'évaluation d'un rapport de telles particules arrêtées dans des zones de capture, le rapport étant égal ou inversement proportionnel aux quantités relatives des analytes d'intérêt dans l'échantillon de fluide.

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