C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 3/06 (2006.01) C12M 1/24 (2006.01) C12M 3/00 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)
Patent
CA 2193810
A cell culture device comprising a container having a cell culture compartment defined by a lower gas permeable film and an upper sheet selectively permeable to compounds of selected sizes and impermeable to cells. The device is adapted to allow culture medium to reside between the upper sheet and the lower gas permeable film. A basal medium compartment is located above the upper sheet and is adapted to allow basal medium to reside upon the upper sheet. Each compartment contains an access port. A gas film support below and in partial contact with the gas permeable film holds the gas permeable film in a substantially horizontal position so that suspension or adherent cells can distribute across the surface of the gas permeable film.
Dispositif de culture cellulaire (10) comprenant un récipient doté d'un compartiment de culture cellulaire (40) délimité par un film inférieur (120), perméable aux gaz, et par une feuille supérieure (20) sélectivement perméable à des composés de tailles sélectionnées. Ce dispositif (10) est conçu pour permettre au milieu de culture (50) de résider entre la feuille supérieure (20) et le film inférieur (120) perméable aux gaz. Un compartiment (30) de milieu basal, situé au-dessus de la feuille supérieure (20), est conçu pour permettre au milieu basal (60) de résider sur cette dernière (20). Chaque compartiment (30, 40) contient un orifice d'accès (70, 90). Un support (130) de film se trouvant au-dessous du film (120) perméable aux gaz et en contact partiel avec lui, maintient ce film (120) en une position sensiblement horizontale de façon à répartir la suspension ou les cellules adhérentes sur toute la surface du film (120) perméable aux gaz.
Wilson John
Wolf Martin L.
Kirby Eades Gale Baker
Wilson John
Wilson Wolf Corporation
Wolf Martin L.
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