G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/542 (2006.01) C12Q 1/04 (2006.01) C12Q 1/28 (2006.01) G01N 33/53 (2006.01) G01N 33/564 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2249778
Disclosed is an assay for an analyte in a fluid test sample such as urine which involves combining the fluid test sample with a reagent system comprising an apo-peroxidase, a redox dye, a hydroperoxide and a metal porpyhrin which is bound to an analyte/analyte specific binding partner which conjugate has a combined molecular weight of at least about 180 K Daltons. When this conjugate interacts with analyte in the fluid test sample, a portion of the specific binding partner is dissociated from the conjugate thereby enabling the metal porpyhrin to reconstitute with the apo-peroxidase. The reconstituted peroxidase can interact with the hydroperoxide and redox dye to provide a colored response to analyte in the fluid test sample.
Corporation Bayer
Osler Hoskin & Harcourt Llp
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