Competitive elisa for the detection of antibodies

G - Physics – 01 – N

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G01N 33/569 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1278259

Abstract of the Disclosure A competitive enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies is disclosed. This ELISA technique is more sensitive, more specific, and more accurate than known ELISA techniques. The com- petitive ELISA of this invention is particularly suited to the detection of human T-cell leukemia-lymphoma virus type III (HTLV-III). An improvement of the ELISA technique represented by the present invention lies in the use of a reaction buffer which suppresses non- specific adsorptive reactions between the test substrate and the test antigen. Furthermore, the ELISA technique is improved by removing contaminants from the target antigen preparation.

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