G - Physics – 01 – N
Patent
G - Physics
01
N
167/41
G01N 23/00 (2006.01) G01N 33/48 (2006.01) G01N 33/78 (2006.01)
Patent
CA 1075153
ABSTRACT OF THE DISCLOSURE The method of this invention is characterized by the use of hydrolyzed cross-linked polyacrylamide particles to which have been bonded, by means of covalent bonds, antibodies against the thyroid hormone to be determined. The particles selected are of a size which forms a stable hydrophyllic suspension. In the preferred embodiment a measured quantity of unextracted human serum is mixed together with a blocking agent in an amount sufficient to displace the thyroid hormone to be measured from thyroxine binding globulin (TBG), a radioactively labeled thyroid hormone of the type to be measured, and the antibody-pol- yacrylamide complex. The hormone to be measured is dis- placed by the blocking agent followed by the competitive binding of the labeled and unlabeled hormone to the anti- body-polyacrylamide particles. The particles are readily separated from the sample liquid and the radioactivity of the particle material and/or in the liquid is determined. Particles containing Alcian yellow or blue dye facilitate practice of the method.
265405
Lewin Nathan
Monthony James F.
Wegfahrt Paul F.
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