Competitive immunoassay using semipermeable membrane

Details Competitive immunoassay using semipermeable membrane Competitive immunoassay using semipermeable membrane

G - Physics

01

N

167/37

G01N 33/74 (2006.01) A61K 9/50 (2006.01) G01N 33/537 (2006.01) F02B 75/02 (2006.01)

Patent

CA 1120397

TEST SET AND METHOD FOR THE DETERMINATION OF FREE SPECIES Abstract of the Disclosure Disclosed is a process for determining the con- centration of an unbound species, e.g., thyroxine, cortisol, testosterone, or digoxin, in a liquid sample containing the species and protein. The sample is incubated with antibody spe- cific to the species to be detected and a distinguishable analo- gue of the species, both of which are separated from the sample by semipermeable membranes capable of excluding the passage of natural protein and antibody but which allow passage of the spe- cies and its analogue. Preferably, the antibody and analogue are contained in semipermeable microcapsules. Free species in the sample permeates the membranes and competes for sites of attach- ment to the antibody with the analogue. The antibody and free species are then separated. The amount of analogue present either in association with the antibody or in the remainder of the reaction system is indicative of the level of free species originally present in the sample. -1-

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