Competitive immunofluorescence assay and test kit

Details Competitive immunofluorescence assay and test kit Competitive immunofluorescence assay and test kit

G - Physics

01

N

167/43

G01N 33/539 (2006.01)

Patent

CA 1218596

COMPETITIVE IMMUNOFLUORESCENCE ASSAY AND TEST KIT Abstract Competitive immunofluorescence assays for antigens in which immune complexes are precipitated with a nonfluorescent, nonlight-scattering precipitant such as polyethylene glycol and the resulting immuno- precipitate is dissolved with a nonfluorescent solvent of low ionic strength that maintains the pH of the solution substantially constant for immunofluorescence intensity reading. The assays are carried out by incubating the sample with fluorecent-labeled antigen, anti-antigen antibody, and a secondary antibody to the anti-antigen antibody followed by addition of the pre- cipitant to form an immunoprecipitate. The precipi- tate is separated by centrifuging, dissolved in the solvent, and the immunofluorescence intensity of the solution is read with a fluorometer and compared to a standard curve.

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