Competitive protein binding assay

Details Competitive protein binding assay Competitive protein binding assay

G - Physics

01

N

150/14, 150/3

G01N 33/68 (2006.01) G01N 33/538 (2006.01) G01N 33/74 (2006.01) G01N 33/82 (2006.01)

Patent

CA 1184100

An improved competitive protein binding assay com- prising; incubating an analyte in the presence of labeled analyte and a protein binder suitable for competitive binding activity by the analyte and the labeled analyte to provide a mixture having free analyte, free labeled analyte, bound analyte and bound labeled analyte, sub- stantially separating the bound analyte and the bound labeled analyte from the free analyte and free labeled analyte to form a first fraction containing substantially the bound analyte and the bound labeled analyte and a second fraction containing substantially the free analyte and the free labeled analyte by causing a predetermined level of the mixture to flow through a bed of an organo- silane coupled to silica gel, and detecting the labeled analyte of at least one of the first and second fractions to determine the concentration of the analyte by compari- son to a reference.

425399

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