Complement-resistant non-mammalian dna viruses and uses thereof

Details Complement-resistant non-mammalian dna viruses and uses thereof Complement-resistant non-mammalian dna viruses and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 15/86 (2006.01) A61K 48/00 (2006.01) C07K 14/705 (2006.01) C12N 7/00 (2006.01) C12N 15/866 (2006.01)

Patent

CA 2375825

Disclosed are methods, nucleic acids, and cells for expressing an exogenous gene in a mammalian cell, involving (i) introducing into the cell a complement- resistant non-mammalian DNA virus (e.g., a baculovirus), optionally having an altered coat protein, the genome of which virus carries an exogenous, gene, and (ii) growingthe cell under conditions such that the gene is expressed.

Cette invention se rapporte à des procédés, des acides nucléiques et des cellules servant à exprimer un gène exogène dans une cellule mammalienne, ces procédés consistant: (i) à introduire dans la cellule un virus d'ADN non mammalien résistant au complément (par exemple un baculovirus), comportant éventuellement une protéine d'enrobage modifiée, le génome de ce virus portant un gène exogène; et (ii) à faire croître cette cellule dans des conditions propres à produire l'expression du gène.

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