C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 7/10 (2006.01) C12N 1/15 (2006.01) C12N 9/36 (2006.01) C12P 1/02 (2006.01)
Patent
CA 2709175
The present invention describes the use of a genetically improved strain of Trichoderma reesei which makes it possible to limit microbiological contaminations during an industrial process. The genetic improvement carried out on the strain enables the latter to overexpress an extracellular protein which has known antimicrobial properties and which is compatible with the secretory system of strains of fungi such as Trichoderma reesei. This modified strain can be used to produce the cellulolytic and/or hemicellulolytic enzymes used in a process for production of ethanol from cellulosic or lignocellulosic materials, said to be second generation.
La présente invention décrit l'utilisation d'une souche de Trichoderma reesei améliorée génétiquement permettant de limiter les contaminations d'ordre microbiologique au cours d'un processus industriel. L'amélioration génétique réalisée sur la souche lui permet de surexprimer une protéine extracellulaire ayant des propriétés anti-microbiennes avérées et compatible avec le système de sécrétion de souches de champignons telles que Trichoderma reesei. Cette souche modifiée petit être utilisée pour produire les enzymes cellulolytiques et/ou hémicellulolytiques utilisées dans un procédé de production d'éthanol à partir de matériaux cellulosiques ou ligno-cellulosiques dit de "seconde génération".
Lopes Ferreira Nicolas
Margeot Antoine
Ifp
Ifp Energies Nouvelles
Robic
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