C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) C12N 1/15 (2006.01) C12P 35/06 (2006.01)
Patent
CA 2093498
Abstract HOE 32/F 099 The complete gene (cefG) encoding the acetyl-CoA: deacetylcephalosporin C acetyltransferase of Cephalosporium acremonium, its isolation and use. The gene (cefG) encoding the acetyl-CoA: deacetylcephalosporin C acetyltransferase (DAC-ATF) of Cephalosporium acremonium (syn. Acremonium chrysogenum) C10 has been isolated. It contains two introns and encodes a protein of 444 amino acids with a molecular weight of 49269 that correlates well with the molecular weight deduced by gel filtration (Mol. weight 52 000 ? 1 000). The cefG gene is linked to the cefEF gene (encoding the bifunctional deacetoxycephalosporin C synthase/hydroxylase) but it is expressed in the opposite orientation to the cefEF gene. The isolated cefG complements the deficiency of deacetylcephalosporin acetyltransferase e.g. in the non-producer mutant C acremonium ATCC 20371 and restores cephalosporin biosynthesis in this strain. The cefG is therefore useful to improve cephalosporin C production.
Fernandez Perrino Francisco J.
Gutierrez Martin Santiago
Martin Martin Juan F.
Velasco Alvarez Javier
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Hoechst Aktiengesellschaft
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