Complex-bound inhibitors of metabolic enzymes capable of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/56 (2006.01) A61K 49/00 (2006.01) G01N 33/86 (2006.01)

Patent

CA 2231354

An inhibitor bound to a substrate of high molecular weight inhibits a metabolic enzyme capable of being activated and is useful as molecular marker to determine the activation of said enzyme and diagnose the presence of said enzyme. Also disclosed is the use of a thrombin inhibitor bound to a substrate of high molecular weight as molecular marker to detect coagulation activation for the purpose of diagnosing coagulation and monitoring a therapeutic treatment. Preferably, the use of dextran-hirudine or PEG-coupled hirudine as molecular marker is disclosed for diagnosing coagulation and monitoring a therapeutic treatment.

L'invention concerne l'utilisation d'un inhibiteur d'une enzyme métabolique activable, lié à un support de poids moléculaire élevé, comme marqueur moléculaire pour déterminer l'activation de l'enzyme en vue de diagnostiquer la présence de ladite enzyme. L'invention concerne en particulier l'utilisation d'un inhibiteur de thrombine lié à un support de poids moléculaire élevé, comme marqueur moléculaire pour la détermination d'une activation de coagulation, lors du diagnostic de la coagulation et du contrôle thérapeutique. L'invention concerne de préférence l'utilisation de dextrane-hirudine ou d'hirudine accouplée au PEG, comme marqueur moléculaire pour le diagnostic de coagulation et le contrôle thérapeutique.

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