Complex formed by a peptide and a major histocompatibility...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/62 (2006.01) A61K 38/00 (2006.01) C07K 14/005 (2006.01) C07K 14/705 (2006.01) C07K 14/74 (2006.01) C07K 16/00 (2006.01) C12N 9/88 (2006.01) C12N 15/73 (2006.01)

Patent

CA 2331061

The invention concerns a micro-organism, preferably a phage, having at its surface a complex consisting of a peptide (P) and a major histocompatibility complex (MHC). More particularly, it concerns a method for expressing a complex formed by a peptide (P) and a major histocompatibility complex (MHC) in a micro-organism so as to produce said complex and present it at a phage surface. The invention also concerns the resulting complexes and their uses for marking, sorting or modulating T cell activity in vitro and in vivo. The invention further concerns the use of the resulting P-MHC complexes for constituting banks of micro-organisms useful for screening and isolating antigenic peptides recognised by T cells.

La présente invention concerne un micro-organisme, préférentiellement un phage, présentant à sa surface un complexe formé d'un peptide (P) et d'un produit du Complexe Majeur d'Histocompatibilité (CMH). Plus particulièrement, la présente invention a pour objet un procédé d'expression d'un complexe formé d'un peptide (P) et d'un produit du Complexe Majeur d'Histocompatibilité (CMH) dans un micro-organisme afin de produire ledit complexe et de le présenter à la surface d'un phage. La présente invention se rapporte encore aux complexes ainsi obtenus et à leur utilisation pour marquer, trier, ou moduler l'activité de cellules T in vitro et in vivo. La présente invention a aussi pour objet l'utilisation des complexes P-CMH obtenus pour la constitution de banques de micro-organismes utiles pour cribler et isoler des peptides antigéniques reconnus par des cellules T.

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