Complexity management and analysis of genomic dna

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2345441

The present invention provides for novel methods of sample preparation and analysis involving reproducibly reducing the complexity of a nucleic sample. The invention further provides for analysis of the above sample by hybridization to an array which may be specifically designed to interrogate the desired fragments for particular characteristics, such as, for example, the presence or absence of a polymorphism. The invention further provides for novel methods of using a computer system to model enzymatic reactions in order to determine experimental conditions before conducting actual experiments.

La présente invention concerne de nouveaux procédés de préparation et d'analyse d'échantillons qui consistent à réduire de manière reproductible la complexité d'un échantillon d'acide nucléique, ainsi que l'analyse de cet échantillon par hybridation sur un réseau qui peut être spécifiquement conçu pour rechercher des caractéristiques particulières dans les fragments désirés, telles que la présence ou l'absence d'un polymorphisme. On décrit également de nouveaux procédés d'utilisation d'un système informatique pour modéliser des réactions enzymatiques en vue de déterminer les conditions expérimentales avant de réaliser véritablement les expériences.

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