Compliant surface multi-well culture plate

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/22 (2006.01) C08J 3/24 (2006.01) C08J 5/00 (2006.01) C12M 1/00 (2006.01) C12M 3/00 (2006.01)

Patent

CA 2702118

A multi-well plate can be loaded with a range of compliant substrates. Commerically- available assays can be used to test cellular responses across a plate with shear modulus from 50 to 51200 Pascals. Cells can be grown in the plates, and can be manipulated and analyzed. Hydrogels can be attached to the bottom of a well. The plates can support the attachment and growth of different cell types and can be compatible with standard 96-well and 384- well plate assays. The mechanical properties of the hydrogels can be reproducible and stable to increase the shelf life of the substrate. The hydrogel can be compatible with growth of a variety of cell types, various attachment ligands such as collagen I, collagen IV, flbronectin, vitronectin, laminin, or RGD peptides and can be coupled to the gel surface.

L'invention concerne une plaque à plusieurs puits pouvant être chargée d'une large gamme de substrats compatibles. On peut utiliser des essais disponibles sur le marché pour tester des réponses cellulaires sur une plaque avec un module de cisaillement allant de 50 à 51 200 Pascals. On peut faire croître des cellules dans les plaques et les manipuler et les analyser. On peut fixer des hydrogels au fond d'un puits. Les plaques peuvent supporter la fixation et la croissance de différents types de cellules et peuvent être compatibles avec des essais sur plaques à 96 puits ou à 384 puits standard. Les propriétés mécaniques des hydrogels peuvent être reproductibles et stables pour augmenter la durée de conservation du substrat. L'hydrogel peut être compatible avec la croissance d'un grand nombre de types de cellules, différents ligands de fixation tels que le collagène I, le collagène IV, la fibronectine, la vitronectine, la laminine ou des peptides RGD et peut être couplé à la surface du gel.

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